Review



ross river virus rrv  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    ATCC ross river virus rrv
    Ross River Virus Rrv, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ross+river+virus+rrv/Ross+River+virus/pmc09685671-44-7-14
    Average 95 stars, based on 87 article reviews
    ross river virus rrv - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Virus:

    Article Title: Development and Validation of a Quantitative, One-Step, Multiplex, Real-Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
    Article Snippet: The concentration of viral RNA or in vitro -transcribed RNA was measured as genome equivalents (GE)/gene copies. .. Genomic RNA preparations from 53 dengue viruses (including 10 strains of DENV-1, 24 strains of DENV-2, 10 strains of DENV-3, and 9 strains of DENV-4), 6 strains of CHIKV, Japanese encephalitis (JE) virus (SA 14-14-2, attenuated vaccine strain), yellow fever (YF) virus (17D, attenuated vaccine strain), Bebaru virus (BEBV) (ATCC VR-600), Ross River virus (RRV) (ATCC VR-373), and Sindbis virus (SBV) (ATCC VR-1248) (see , , and ) were obtained from BEI Resources-ATCC (Manassas, VA). .. DENV laboratory strains and JE and YF virus vaccine strains were received from the Walter Reed Army Institute of Research (WRAIR) (Silver Spring, MD), CHIKV R-91142, CHIKV S-27, CHIKV SL-15649, and all other DENV strains were received from BEI Resources-ATCC, the CHIKV proficiency panel was obtained from CDC (Fort Collins, CO), and CHIKV 181-25 (attenuated vaccine strain) was received from Shuenn-Jue Wu (NMRC, MD). table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Virus a GE/assay No. of replicates b C T value Mean SD % CV DENV-3 5 × 10 6 24 17.84 0.26 1.5 5 × 10 5 24 21.08 0.34 1.6 5 × 10 4 24 24.43 0.35 1.4 5 × 10 3 24 27.70 0.39 1.4 5 × 10 2 24 31.17 0.50 1.6 5 × 10 1 24 34.55 0.72 2.1 CHIKV 6 × 10 6 24 18.08 0.28 1.5 6 × 10 5 24 21.30 0.31 1.5 6 × 10 4 24 24.69 0.38 1.5 6 × 10 3 24 28.14 0.38 1.3 6 × 10 2 24 31.36 0.80 2.5 6 × 10 1 24 34.59 1.10 3.2 Open in a separate window a The multiplex RT-PCR was carried out on RNAs extracted from DENV and CHIKV in vitro transcripts. b Quadruplicate runs over 3 days were carried out by two different operators.

    Article Title: Development and application of an indirect ELISA for detecting equine IgG antibodies against Getah virus with recombinant E2 domain protein
    Article Snippet: .. The ascitic fluids from mice immunized with ross river virus (RRV) were purchased from ATCC (United States). ..

    Article Title: Identification and Characterization of a Ross River Virus Variant That Grows Persistently in Macrophages, Shows Altered Disease Kinetics in a Mouse Model, and Exhibits Resistance to Type I Interferon
    Article Snippet: .. Ross River virus (RRV) derived from an infectious clone of strain T48 (originally designated RR64) ( 25 ) was used to initially infect the mouse macrophage cell line RAW 264.7 (ATCC TIB-71) cultured at 5.0 × 10 5 cells per well/ml in 24-well trays (Nunc, Roskilde, Denmark). ..

    Article Title: Development of an enzyme-linked immunosorbent assay for Getah virus infection in horses using recombinant E2 protein as an antigen.
    Article Snippet: Getah virus causes fever, skin eruptions, and limb edema in horses.. For a high-throughput and time-saving method for serodiagnosis, we explored immunogenic antigens of Getah virus, and established an enzyme-linked immunosorbent assay (ELISA) using a recombinant protein.. Western blot analysis using sera from infected horses showed strong reaction with viral antigens around 46 kDa corresponding to E1 or E2 glycoproteins.

    Article Title: A potent neutralizing IgM mAb targeting the N218 epitope on E2 protein protects against Chikungunya virus pathogenesis
    Article Snippet: .. CHIKV Ross (GenBank Accession number: {"type":"entrez-nucleotide","attrs":{"text":"AF490259","term_id":"323516040","term_text":"AF490259"}} AF490259 ), kindly provided by Dr. Ooi Eng Eong from DUKE-NUS, Sindbis virus (SINV) (GenBank Accession number: {"type":"entrez-nucleotide","attrs":{"text":"NC_001547","term_id":"9790313","term_text":"NC_001547"}} NC_001547 ) and Ross River Virus (RRV) (ATCC) were also used in the PRNT. ..

    Article Title: A CRISPR Activation Screen Identifies an Atypical Rho GTPase That Enhances Zika Viral Entry
    Article Snippet: .. GFP-expressing alphaviruses (Sindbis virus (SINV), o’nyong’nyong virus (ONNV), Ross River virus (RRV), and Venezuelan equine encephalitis virus (VEEV)) were generated in baby hamster kidney 21 cells (BHK-21; ATCC) as previously described [ , ]. ..

    Derivative Assay:

    Article Title: Identification and Characterization of a Ross River Virus Variant That Grows Persistently in Macrophages, Shows Altered Disease Kinetics in a Mouse Model, and Exhibits Resistance to Type I Interferon
    Article Snippet: .. Ross River virus (RRV) derived from an infectious clone of strain T48 (originally designated RR64) ( 25 ) was used to initially infect the mouse macrophage cell line RAW 264.7 (ATCC TIB-71) cultured at 5.0 × 10 5 cells per well/ml in 24-well trays (Nunc, Roskilde, Denmark). ..

    Cell Culture:

    Article Title: Identification and Characterization of a Ross River Virus Variant That Grows Persistently in Macrophages, Shows Altered Disease Kinetics in a Mouse Model, and Exhibits Resistance to Type I Interferon
    Article Snippet: .. Ross River virus (RRV) derived from an infectious clone of strain T48 (originally designated RR64) ( 25 ) was used to initially infect the mouse macrophage cell line RAW 264.7 (ATCC TIB-71) cultured at 5.0 × 10 5 cells per well/ml in 24-well trays (Nunc, Roskilde, Denmark). ..

    Western Blot:

    Article Title: Development of an enzyme-linked immunosorbent assay for Getah virus infection in horses using recombinant E2 protein as an antigen.
    Article Snippet: Getah virus causes fever, skin eruptions, and limb edema in horses.. For a high-throughput and time-saving method for serodiagnosis, we explored immunogenic antigens of Getah virus, and established an enzyme-linked immunosorbent assay (ELISA) using a recombinant protein.. Western blot analysis using sera from infected horses showed strong reaction with viral antigens around 46 kDa corresponding to E1 or E2 glycoproteins.

    Plaque Reduction Neutralization Test:

    Article Title: A potent neutralizing IgM mAb targeting the N218 epitope on E2 protein protects against Chikungunya virus pathogenesis
    Article Snippet: .. CHIKV Ross (GenBank Accession number: {"type":"entrez-nucleotide","attrs":{"text":"AF490259","term_id":"323516040","term_text":"AF490259"}} AF490259 ), kindly provided by Dr. Ooi Eng Eong from DUKE-NUS, Sindbis virus (SINV) (GenBank Accession number: {"type":"entrez-nucleotide","attrs":{"text":"NC_001547","term_id":"9790313","term_text":"NC_001547"}} NC_001547 ) and Ross River Virus (RRV) (ATCC) were also used in the PRNT. ..

    Generated:

    Article Title: A CRISPR Activation Screen Identifies an Atypical Rho GTPase That Enhances Zika Viral Entry
    Article Snippet: .. GFP-expressing alphaviruses (Sindbis virus (SINV), o’nyong’nyong virus (ONNV), Ross River virus (RRV), and Venezuelan equine encephalitis virus (VEEV)) were generated in baby hamster kidney 21 cells (BHK-21; ATCC) as previously described [ , ]. ..



    Similar Products

    95
    ATCC ross river virus rrv
    Ross River Virus Rrv, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ross+river+virus+rrv/Ross+River+virus/pmc09685671-44-7-14
    Average 95 stars, based on 1 article reviews
    ross river virus rrv - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    ATCC mouse virulent rrv prototype strain t48
    Mouse Virulent Rrv Prototype Strain T48, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ross+river+virus+rrv/Ross+River+virus/pm36569444-453-9-14
    Average 95 stars, based on 1 article reviews
    mouse virulent rrv prototype strain t48 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    95
    ATCC rrv prototype strain t48
    Rrv Prototype Strain T48, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ross+river+virus+rrv/Ross+River+virus/pmc09768462-446-10-14
    Average 95 stars, based on 1 article reviews
    rrv prototype strain t48 - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    rrv e2  (ATCC)
    91
    ATCC rrv e2
    TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or <t>RRV</t> (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV <t>E2</t> and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.
    Rrv E2, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ross+river+virus+rrv/Ross+River+virus+immune+ascitic+fluid/pmc09503900-78-12-14
    Average 91 stars, based on 1 article reviews
    rrv e2 - by Bioz Stars, 2026-09
    91/100 stars
      Buy from Supplier

    95
    ATCC rrv ross river virus mcb master cell bank atcc american type culture collection
    TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or <t>RRV</t> (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV <t>E2</t> and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.
    Rrv Ross River Virus Mcb Master Cell Bank Atcc American Type Culture Collection, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ross+river+virus+rrv/Ross+River+virus/us11357846-178-168-176
    Average 95 stars, based on 1 article reviews
    rrv ross river virus mcb master cell bank atcc american type culture collection - by Bioz Stars, 2026-09
    95/100 stars
      Buy from Supplier

    91
    ATCC anti rrv mouse ascites fluid
    TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or <t>RRV</t> (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV <t>E2</t> and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.
    Anti Rrv Mouse Ascites Fluid, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ross+river+virus+rrv/Ross+River+virus+immune+ascitic+fluid/pmc07666055-743-33-37
    Average 91 stars, based on 1 article reviews
    anti rrv mouse ascites fluid - by Bioz Stars, 2026-09
    91/100 stars
      Buy from Supplier

    91
    ATCC rrv mouse ascites fluid
    TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or <t>RRV</t> (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV <t>E2</t> and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.
    Rrv Mouse Ascites Fluid, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ross+river+virus+rrv/Ross+River+virus+immune+ascitic+fluid/pmc07666055-766-12-18
    Average 91 stars, based on 1 article reviews
    rrv mouse ascites fluid - by Bioz Stars, 2026-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or RRV (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV E2 and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.

    Journal: Vaccines

    Article Title: A Bivalent Trans-Amplifying RNA Vaccine Candidate Induces Potent Chikungunya and Ross River Virus Specific Immune Responses

    doi: 10.3390/vaccines10091374

    Figure Lengend Snippet: TR-RNA amplification by the CHIKV replicase. HEK 293T cells were transfected with 2 μg of the replicase RNA or TR-luc-RNA as irrelevant RNA together with 0.5 μg of the indicated TR-RNAs. The total amount of TR-RNA was kept constant between the single and double transfections. For comparison, cells were infected with CHIKV or RRV (MOI 3). RNA was harvested after 6 h, 16 h, and 24 h, and ( A ): CHIKV E2 and ( B ): RRV E2 RNA levels were measured by RT-qPCR. Numbers indicate the fold change in TR-RNA amount 24 h after co-transfection with the replicase. Ct values, which were below the cutoff, were set to 0.1 for plotting. Data are mean values ± SEM of three independent experiments.

    Article Snippet: Primary antibodies were directed against CHIKV E2 (Eurogentec, Cologne, Germany; custom made), RRV E2 (ATCC VR-1246AF), and β-actin (Sigma, Munich, Germany; no. A5441).

    Techniques: RNA Amplification, Transfection, Comparison, Infection, Quantitative RT-PCR, Cotransfection

    Antigen expression from TR-RNAs. ( A ): CHIKV E2 and RRV E2 protein expression in cellular lysates 6 h and 24 h after transfection of 2 μg of replicase-RNA together with 0.5 μg of the indicated TR-RNAs. As control, cells were infected with CHIKV or RRV (MOI 3) or left untreated (ctrl). ( B ): Protein expression 48 h after transfection of 8 μg of the replicase-RNA with 2 μg of the indicated TR-RNAs in cellular lysates or concentrated supernatants. The depicted Western blots are representative of three independent experiments. Uncropped blots and densitometry readings are given in .

    Journal: Vaccines

    Article Title: A Bivalent Trans-Amplifying RNA Vaccine Candidate Induces Potent Chikungunya and Ross River Virus Specific Immune Responses

    doi: 10.3390/vaccines10091374

    Figure Lengend Snippet: Antigen expression from TR-RNAs. ( A ): CHIKV E2 and RRV E2 protein expression in cellular lysates 6 h and 24 h after transfection of 2 μg of replicase-RNA together with 0.5 μg of the indicated TR-RNAs. As control, cells were infected with CHIKV or RRV (MOI 3) or left untreated (ctrl). ( B ): Protein expression 48 h after transfection of 8 μg of the replicase-RNA with 2 μg of the indicated TR-RNAs in cellular lysates or concentrated supernatants. The depicted Western blots are representative of three independent experiments. Uncropped blots and densitometry readings are given in .

    Article Snippet: Primary antibodies were directed against CHIKV E2 (Eurogentec, Cologne, Germany; custom made), RRV E2 (ATCC VR-1246AF), and β-actin (Sigma, Munich, Germany; no. A5441).

    Techniques: Expressing, Transfection, Control, Infection, Western Blot